Active Recombinant Full Legnth Mouse Uchl1 Protein, His-tagged

• Cat.No.: Uchl1-7361M
• Product Overview: Recombinant mouse Uchl1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Liquid
• Molecular Mass: 27.2 kDa
• Protein length: 1-223 a.a.
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMGSMQLKPMEINPEMLNKVLAKLGVAGQWRFADVLGLEEETLGSVPSPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLEFEDGSVLKQFLSETEKLSPEDRAKCFEKNEAIQAAHDSVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDSLLQDAAKVCREFTEREQGEVRFSAVALCKAA
• Purity: > 90 % by SDS-PAGE
• Stability: Shelf life: one year from despatch.
• Storage: Store undiluted at 2-8 centigrade for one week or (in aliquots) at -20 to -80 centigrade for longer. Avoid repeated freezing and thawing.
• Concentration: 1.0 mg/mL (determined by bradford assay)
• Storage Buffer: Phosphate buffered saline containing 10 % glycerol, 1 mM DTT.
• Bio-activity: Specific activity is > 80pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0pmole of ubiquitin-AMC per minute at pH 7.5, at 37C

Gene Information

• Gene Name: Uchl1 ubiquitin carboxy-terminal hydrolase L1 [ Mus musculus (house mouse) ]
• Official Symbol: Uchl1
• Synonyms: Uchl1; ubiquitin carboxy-terminal hydrolase L1; gad; PGP9; C88048; PGP9.5; R75593; UCH-L1; UCHL-1; PGP 9.5; AW822034; ubiquitin carboxyl-terminal hydrolase isozyme L1; gracile axonal dystrophy; neuron cytoplasmic protein 9.5; protein gene product 9.5; ubiquitin thioesterase L1; EC 3.4.19.12
• Gene ID: 22223
• mRNA Refseq: NM_011670
• Protein Refseq: NP_035800
• UniProt ID: Q9R0P9