Active Recombinant Human UCHL1 protein, His-tagged

• Cat.No.: UCHL1-8938H
• Product Overview: Human Ubiquitin Carboxy-terminal Hydrolase (UCHL1), also known as PGP9.5, with N-terminal His-tag, expressed in E. coli expression system.

Specification

• Description: The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Aqueous buffer solution.
• Bio-activity: 7433 pmol/min/μg
• Molecular Mass: 25.8 kDa
• Protein length: 2-223
• Purity: >90%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: At least 6 months at -80 centigrade.
• Concentration: 2.38 mg/ml
• Storage Buffer: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.
• Shipping: At -80.

Gene Information

• Gene Name: UCHL1 ubiquitin C-terminal hydrolase L1 [ Homo sapiens (human) ]
• Official Symbol: UCHL1
• Synonyms: UCHL1; ubiquitin C-terminal hydrolase L1; NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53; ubiquitin carboxyl-terminal hydrolase isozyme L1; epididymis luminal protein 117; epididymis secretory protein Li 53; neuron cytoplasmic protein 9.5; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin thioesterase L1; ubiquitin thiolesterase; EC 3.4.19.12
• Gene ID: 7345
• mRNA Refseq: NM_004181
• Protein Refseq: NP_004172
• MIM: 191342
• UniProt ID: P09936