Active Recombinant Equine Interleukin 6 (Interferon, Beta 2)

• Cat.No.: IL6-258E
• Product Overview: Recombinant Equine IL-6 was expressed inYeast. MW= 20.6 kDa (181 aa).

Specification

• Species: Equine
• Source: Yeast
• Tag: Non
• Description: Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. In terms of host response to a foreign pathogen, IL-6 has been shown, in mice, to be required for resistance against the bacterium, Streptococcus pneumoniae. IL-6 is also a "myokine," a cytokine produced from muscle, and is elevated in response to muscle contraction.
• Formulation/ Reconstitution: Lyophilized. Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
• Purification: Ion-exchange chromatography.
• Purity: >95% as visualized by SDS-PAGE analysis.
• Amino Acid Sequence: TPLPLGEDET TSNGPLLTTA DKTKQHIKYI LGKISALKNE MCNNFSKCEN SKEVLAENNL NLPKMAEKDG CFQSGFNQET CLMKITTGLS EFQIYLEYLQ NEFKGEKENI KTMQISTKVL VQILMQKMKN PEVTTPDPTA KSSLLAKLHS QNEWLKNTTT HLILRSLEDF LQFSLRAVRI M.
• Bioactivity: The biological activity of recombinant equine IL-6 was measured in a cell proliferation assay using the mouse B9 cell line. The ED50 for this effect is typically0.05 – 1.0 ng/mL.
• Stability And Storage: Stable for up to twelve months from date of receipt at -20°C. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: IL6 interleukin 6 (interferon, beta 2) [ Equus caballus ]
• Synonyms: interleukin 6 (interferon, beta 2); IL6; IL-6; interleukin-6; interleukin 6
• Gene ID: 100034196
• mRNA Refseq: NM_001082496.1
• Protein Refseq: NP_001075965.1
• UniProt ID: Q95181
• Chromosome Location: 4
• Pathway: Cytokine-cytokine receptor interaction; Cytosolic DNA-sensing pathway; Graft-versus-host disease; Hematopoietic cell lineage; Hypertrophic cardiomyopathy (HCM); Intestinal immune network for IgA production; Jak-STAT signaling pathway; NOD-like receptor signaling pathway; Pathways in cancer; Prion diseases; Toll-like receptor signaling pathway