Active Recombinant Dog CYP1B1 Protein

Cat.No. : CYP1B1-120D
Product Overview : Recombinant dog CYP1B1 protein without tag were coexpressed in E. coli.
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Species : Dog
Source : E.coli
Description : Cytochrome P450 1B1 (CYP1B1), a member of CYP superfamily, is expressed in liver and extrahepatic tissues carries out the metabolism of numerous xenobiotics, including metabolic activation of polycyclic aromatic hydrocarbons.
Bio-activity : Cytochrome c Reductase Activity: 1107 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: 7-ethoxyresorufin O-deethylase
Linear with time up to: 90 sec.(Determined at 0.3 µM substrate, using a P450 concentration of 1 pmol/ml)
Linear with P450 up to: 2.5 pmol/ml(Determined at 0.3 µM substrate, using an incubation time of 10 sec)
Vmax: 39 pmol/min/pmol CYP
Km: 0.03 μM
Usage : For laboratory (research) purposes only
Storage : Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration : P450 Concentration: 6.3 nmol/ml
Protein Concentration: 13.1 mg/ml
Specific P450 Content: 481 pmol/mg protein
Storage Buffer : 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Gene Name CYP1B1 cytochrome P450 family 1 subfamily B member 1 [ Canis lupus familiaris (dog) ]
Official Symbol CYP1B1
Synonyms CYP1B1; cytochrome P450 family 1 subfamily B member 1; cytochrome P450 1B1; cytochrome P450, family 1, subfamily B, polypeptide 1
Gene ID 483038
mRNA Refseq NM_001159684
Protein Refseq NP_001153156
UniProt ID C1KG39

Not For Human Consumption!

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