Active Recombinant Dog CYP1B1 Protein
Cat.No. : | CYP1B1-120D |
Product Overview : | Recombinant dog CYP1B1 protein without tag were coexpressed in E. coli. |
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Species : | Dog |
Source : | E.coli |
Description : | Cytochrome P450 1B1 (CYP1B1), a member of CYP superfamily, is expressed in liver and extrahepatic tissues carries out the metabolism of numerous xenobiotics, including metabolic activation of polycyclic aromatic hydrocarbons. |
Bio-activity : | Cytochrome c Reductase Activity: 1107 nmol/min/mg protein P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase]) Activity: 7-ethoxyresorufin O-deethylase Linear with time up to: 90 sec.(Determined at 0.3 µM substrate, using a P450 concentration of 1 pmol/ml) Linear with P450 up to: 2.5 pmol/ml(Determined at 0.3 µM substrate, using an incubation time of 10 sec) Vmax: 39 pmol/min/pmol CYP Km: 0.03 μM |
Usage : | For laboratory (research) purposes only |
Storage : | Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice. |
Concentration : | P450 Concentration: 6.3 nmol/ml Protein Concentration: 13.1 mg/ml Specific P450 Content: 481 pmol/mg protein |
Storage Buffer : | 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA |
Gene Name | CYP1B1 cytochrome P450 family 1 subfamily B member 1 [ Canis lupus familiaris (dog) ] |
Official Symbol | CYP1B1 |
Synonyms | CYP1B1; cytochrome P450 family 1 subfamily B member 1; cytochrome P450 1B1; cytochrome P450, family 1, subfamily B, polypeptide 1 |
Gene ID | 483038 |
mRNA Refseq | NM_001159684 |
Protein Refseq | NP_001153156 |
UniProt ID | C1KG39 |
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Not For Human Consumption!
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