Active Recombinant Dog CYP1B1 Protein

• Cat.No.: CYP1B1-120D
• Product Overview: Recombinant dog CYP1B1 protein without tag were coexpressed in E. coli.

Specification

• Species: Dog
• Source: E.coli
• Description: Cytochrome P450 1B1 (CYP1B1), a member of CYP superfamily, is expressed in liver and extrahepatic tissues carries out the metabolism of numerous xenobiotics, including metabolic activation of polycyclic aromatic hydrocarbons.
• Bio-activity: Cytochrome c Reductase Activity: 1107 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: 7-ethoxyresorufin O-deethylase
  Linear with time up to: 90 sec.(Determined at 0.3 µM substrate, using a P450 concentration of 1 pmol/ml)
  Linear with P450 up to: 2.5 pmol/ml(Determined at 0.3 µM substrate, using an incubation time of 10 sec)
  Vmax: 39 pmol/min/pmol CYP
  Km: 0.03 μM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 6.3 nmol/ml
  Protein Concentration: 13.1 mg/ml
  Specific P450 Content: 481 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP1B1 cytochrome P450 family 1 subfamily B member 1 [ Canis lupus familiaris (dog) ]
• Official Symbol: CYP1B1
• Synonyms: CYP1B1; cytochrome P450 family 1 subfamily B member 1; cytochrome P450 1B1; cytochrome P450, family 1, subfamily B, polypeptide 1
• Gene ID: 483038
• mRNA Refseq: NM_001159684
• Protein Refseq: NP_001153156
• UniProt ID: C1KG39