Active Recombinant Cynomolgus TSLP protein, His-tagged

• Cat.No.: TSLP-0773C
• Product Overview: Recombinant Cynomolgus TSLP protein(Tyr29-Gln159(R127A,R130S)), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human TSLP R, Fc Tag at 5 μg/mL (100 μL/well) can bind Cynomolgus TSLP (R127A, R130S) Protein, His Tag with a linear range of 1-4 ng/mL. Immobilized Cynomolgus TSLP (R127A, R130S) Protein, His Tag at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human TSLP Antibody, Human IgG2 with a linear range of 0.5-8 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 16.9 kDa. The protein migrates as 21-30 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Tyr29-Gln159(R127A,R130S)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 29-159 a.a.