Active Recombinant Human TSLP Protein, His-tagged, R127A, R130A

• Cat.No.: TSLP-139H
• Product Overview: Recombinant Human TSLP protein (Q969D9) (Tyr29-Gln159, with mutation R127A and R130A) is produced by HEK293 Cells expression system. This protein is fused with a His tag at the C-terminus. It is identical to TSLP with mutation R127A and R130A in the reference.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human TSLP R, Fc Tag at 5 μg/mL (100 μL/well) can bind Human TSLP (R127A, R130A) with a linear range of 0.8-6 ng/mL.
  Immobilized Human TSLP (R127A, R130A) at 1 μg/mL (100 μL/well) can bind Anti-TSLP, Human IgG2 with a linear range of 0.4-3 ng/mL.
• Molecular Mass: The protein has a calculated MW of 16.7 kDa. The protein migrates as 22-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Tyr29-Gln159
• Endotoxin: < 1.0 EU per μg by the LAL method.
• Purity: > 90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.

Gene Information

• Gene Name: TSLP thymic stromal lymphopoietin [ Homo sapiens ]
• Official Symbol: TSLP
• Synonyms: TSLP; thymic stromal lymphopoietin
• Gene ID: 85480
• mRNA Refseq: NM_033035
• Protein Refseq: NP_149024
• MIM: 607003
• UniProt ID: Q969D9