Active Recombinant Cynomolgus TP53 Protein, Alexa Fluor 555 conjugated

• Cat.No.: TP53-1162CAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Cynomolgus (Macaca fascicularis) p53 (E3U906) (Met 1-Asp 393) was expressed and purified.

Specification

• Species: Monkey
• Source: E.coli
• Protein Length: 393
• Form: Lyophilized
• Bio-activity: 1. Measured by its ability to bind biotinylated human S100B in a functional ELISA.
  2. Measured by its ability to bind biotinylated human RRM2B in a functional ELISA.
• Molecular Mass: The recombinant Cynomolgus p53 consisting of 393 amino acids and has a calculated molecular mass of 43.7 kDa. It migrates as an approximately 48 kDa band in SDS-PAGE under reducing conditions.
• N-terminal Sequence Analysis: Met 1
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 88 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 100 mM Tris, 3% glycerol, 4 mM DTT, pH 8.01, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: TP53 tumor protein p53 [ Macaca fascicularis ]
• Official Symbol: TP53
• Synonyms: p53; Tumor suppressor p53
• Gene ID: 102135998
• mRNA Refseq: NM_001287679
• Protein Refseq: NP_001274608
• UniProt ID: P56423