Active Recombinant Cynomolgus/Rhesus TNF protein

• Cat.No.: TNF-1168C
• Product Overview: Recombinant Cynomolgus/Rhesus TNF protein(Val77-Leu233) was expressed in E. coli.

Specification

• Species: Cynomolgus/Rhesus
• Source: E.coli
• Tag: Non
• ProteinLength: Val77-Leu233
• Form: Lyophilized from sterile 50 mM Tris, 50 mM NaCl, pH 8.0.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 2-20 pg/mL.
• Molecular Mass: The recombinant cynomolgus / rhesus TNF consists of 158 amino acids and has a calculated molecular mass of 17.4 kDa.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.