Recombinant Mouse TNF-alpha Soluble, FLAG-tagged

• Cat.No.: TNF-64M
• Product Overview: The extracellular domain of mouse tumor necrosis factor-α (TNF-α) (amino acids 77-235) is fused at the N-terminus to an 8 amino acid linker peptide and a FLAG tag.

Specification

• Species: Mouse
• Tag: Flag
• Protein Length: 77-235 a.a.
• Form: Lyophilized powder containing 50 μg recombinant TNF-α and PBS, 1 mg/mL TNF-α after reconstitution.
• Molecular Mass: Molecular weight ~20 kDa by SDS-PAGE.
• Endotoxin: < 0.1="" eu/μg="" purified="" protein="" (lal="" test;="" bio="">Whittaker).
• Purity: ≥ 95% as determined by SDS-PAGE.
• Characteristic: ED50: 0.02 ng/mL (WEHI 164 cells). TNF-α binds to human, mouse and rat TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer, TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane bound TNF-α.
• Applications: Induction of TNF-R1 and TNF-R2 mediated responses. Induction of TNF-R2 mediated responses requires addition of a secondary cross-linking anti-FLAG antibody.Inducing Apoptosis: Recombinant mouse TNF-α protein induces apoptosis in WEHI 164 and other TNF-α sensitive cells. Optimal concentration varies with cell type and should be determined by testing serial dilutions on cells (For WEHI 164 cells use 0.5-1 ng/mL). The optimal dilution for a specific application should be determined by the researcher.
• Usage: For in vitro research use only. Not for use in diagnostics or in humans.
• Storage: Store at -20°C. Aliquot solutions to avoid repeated freeze/thaw. Lyophilized product is stable at -20°C for at least 6 months.
• Reconstitution: Prepare a 1 mg/mL stock solution by dissolving the contents of the vial in 50 μL of sterile H20. Further dilutions should be made with medium containing 5% fetal calf serum.
• Warning: No warranties, expressed or implied, are made regarding the use of this product. Creative Biomart is not liable for any damage, personal injury, or economic loss caused by this product.

Gene Information

• Gene Name: Tnf tumor necrosis factor [ Mus musculus ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; TNF-a; TNF alpha; cachectin; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434
• Gene ID: 21926
• mRNA Refseq: NM_013693
• Protein Refseq: NP_038721
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem
• Function: cytokine activity; cytokine activity; identical protein binding; protease binding; protease binding; protein binding; transcription regulatory region DNA binding; tumor necrosis factor receptor binding; tumor necrosis factor receptor binding; tumor necrosis factor receptor binding; tumor necrosis factor receptor binding