Active Recombinant Cynomolgus/Rhesus TFRC protein, His-tagged

• Cat.No.: TFRC-4685R
• Product Overview: A DNA sequence encoding the Cynomolgus/Rhesus TFRC (NP_001244232.1)(Cys89-Phe760) was expressed with a polyhistidine tag at the N-terminus.

Specification

• Species: Cynomolgus/Rhesus
• Source: HEK293
• Tag: His
• Protein Length: Cys89-Phe760
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to neutralize transferrin-mediated proliferation of MCF-7 cells. The ED50 for this effect is typically 0.2-2μg/mL.
• Molecular Mass: The recombinant cynomolgus/rhesus TFRC comprises 691 amino acids and has a calculated molecular mass of 77.5 KDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.