Active Recombinant Canine FCGRT&B2M protein, His-Avi-tagged, Biotinylated

• Cat.No.: FCGRT&B2M-1417C
• Product Overview: Biotinylated Recombinant Canine FCGRT&B2M protein(Ala23-Pro298|Val28-Asn125), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: Avi&His
• Protein Length: 23-298;28-125 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Monoclonal Anti-Human CTLA-4 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Canine FcRn / FCGRT&B2M Heterodimer, His,Avitag&Tag Free with a linear range of 0.039-2.5 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 28.3 kDa | 11.5 kDa. The protein migrates as ＜14.4 kDa and 38-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala23-Pro298|Val28-Asn125
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.