Active Recombinant Cynomolgus PDCD1LG2 protein, His-tagged

• Cat.No.: PDCD1LG2-1218C
• Product Overview: Recombinant Cynomolgus PDCD1LG2 protein(A4GW30)(Met1-Pro219), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus PDCD1LG2-His at 10 μg/ml (100 μl/well) can bind human PD1-Fc, The EC50 of human PD1-Fch is 0.09-0.21 μg/ml.
• Molecular Mass: The recombinant cynomolgus PDCD1LG2 comprises 211 amino acids and has a calculated molecular mass of 24 KDa. The apparent molecular mass of it is approximately 37-43 KDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-Pro219
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.