Active Recombinant Cynomolgus CYP2C19 Protein

• Cat.No.: CYP2C19-126C
• Product Overview: Recombinant cynomolgus CYP2C19 protein and human CYP-reductase without tag were coexpressed in E. coli.

Specification

• Species: Cynomolgus
• Source: E.coli
• Description: Proton Pump inhibitors (PPIs) are commonly used for a variety of acid related disorders. Despite the overall effectiveness and safety profile of PPIs, some patients do not respond adequately or develop treatment related adverse events. This variable response among patients is in part due to genotype variability of CYP2C19, the gene encoding the CYP450 (CYP2C19) isoenzyme responsible for PPIs metabolism.
• Bio-activity: Cytochrome c Reductase Activity: 154 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Tolbutamide 4-hydroxylase
  Linear with time up to: 30 min(Determined at 350 µM substrate, using a P450 concentration of 100 pmol/ml)
  Linear with P450 up to: 100 pmol/ml(Determined at 350 µM substrate, using an incubation time of 20 min)
  Vmax: 1.5 pmol/min/pmol CYP
  Km: 437 μM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 2.2 nmol/ml
  Protein Concentration: 12.3 mg/ml
  Specific P450 Content: 179 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP2C19 cytochrome P450 family 2 subfamily C member 19 [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: CYP2C19
• Synonyms: CYP2C19; cytochrome P450 family 2 subfamily C member 19; CYP2C75; cytochrome P450 2C9-like; cytochrome P450 family 2 subfamily C member 19; cytochrome P450 2C75; cytochrome P450, family 2, subfamily C, polypeptide 19
• Gene ID: 102147103
• mRNA Refseq: NM_001283290
• Protein Refseq: NP_001270219
• UniProt ID: Q0HA19