Active Recombinant Cynomolgus CD3E & CD3D protein

Cat.No. : CD3E & CD3D-2972C
Product Overview : Recombinant Cynomolgus CD3E&CD3D protein(Gln 22-Asp 117 (CD3E) & Phe 22-Ala 105 (CD3D)) was expressed in HEK293.
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Species : Cynomolgus
Source : HEK293
Tag : Non
Protein Length : Gln 22-Asp 117 (CD3E) & Phe 22-Ala 105 (CD3D)
Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin at 1 μg/mL (100 μL/well) can bind Mouse Anti-Human CD3 (SP34-2) (mouse IgG1) with a linear range of 2-16 ng/mL.Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
Molecular Mass : Cynomolgus CD3E & CD3D Heterodimer Protein, Llama IgG2b Fc Tag & Llama IgG2b Fc Tag, low endotoxin is produced by co-expression of CD3E and CD3D, has a calculated MW of 41.9 kDa (CD3E) and 40.8 kDa (CD3D). Subunit CD3E is fused with a llama IgG2b Fc tag at the C-terminus and subunit CD3D is fused with a llama IgG2b Fc tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) & Phe 22 (CD3D). The reducing (R) protein migrates as 50 kDa and 55-65 kDa due to glycosylation.
Endotoxin : Less than 0.01 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Not For Human Consumption!

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