Active Recombinant Human CD3E & CD3D protein, His-tagged

Cat.No. : CD3E & CD3D-376H
Product Overview : Recombinant Human CD3 epsilon & CD3 delta Heterodimer Protein, Asp 23 - Asp 126 (CD3E, P07766) and & Phe 22 - Ala 105 (CD3D, P04234), was produced in human 293 cells (HEK293).
Availability February 22, 2025
Unit
Price
Qty
  • Specification
  • Gene Information
  • Related Products
  • Download
Species : Human
Source : HEK293
Tag : His
Protein Length : Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with a linear range of 0.2-6 ng/mL.
Molecular Mass : The protein has a calculated MW of 16.6 kDa (CD3E) and 13.4 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag. The reducing (R) heterodimer protein migrates as 25-30 kDa (CD3E) and 20 kDa and 22 kDa (CD3D) due to glycosylation.
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
Official Symbol CD3E
Synonyms CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683;
Gene ID 916
mRNA Refseq NM_000733
Protein Refseq NP_000724
UniProt ID P07766

Not For Human Consumption!

Inquiry

  • Reviews
  • Q&As

Customer Reviews (0)

Write a review

Q&As (0)

Ask a question

Ask a Question for All CD3E;CD3D Products

Required fields are marked with *

My Review for All CD3E;CD3D Products

Required fields are marked with *

0

Inquiry Basket

cartIcon