Active Recombinant Human CD3E & CD3D protein, His-tagged
Cat.No. : | CD3E & CD3D-376H |
Product Overview : | Recombinant Human CD3 epsilon & CD3 delta Heterodimer Protein, Asp 23 - Asp 126 (CD3E, P07766) and & Phe 22 - Ala 105 (CD3D, P04234), was produced in human 293 cells (HEK293). |
Availability | February 22, 2025 |
Unit | |
Price | |
Qty |
- Specification
- Gene Information
- Related Products
- Download
Species : | Human |
Source : | HEK293 |
Tag : | His |
Protein Length : | Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with a linear range of 0.2-6 ng/mL. |
Molecular Mass : | The protein has a calculated MW of 16.6 kDa (CD3E) and 13.4 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag. The reducing (R) heterodimer protein migrates as 25-30 kDa (CD3E) and 20 kDa and 22 kDa (CD3D) due to glycosylation. |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 centigrade to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name | CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ] |
Official Symbol | CD3E |
Synonyms | CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683; |
Gene ID | 916 |
mRNA Refseq | NM_000733 |
Protein Refseq | NP_000724 |
UniProt ID | P07766 |
◆ Recombinant Proteins | ||
CD3E & CD3D-109H | Recombinant Human CD3E & CD3D protein, hFc-tagged | +Inquiry |
CD3E&CD3D-219H | Active Recombinant Human CD3E&CD3D, His&flag-tagged | +Inquiry |
CD3E & CD3D-401M | Recombinant Mouse CD3E & CD3D protein, hFc-tagged | +Inquiry |
CD3E & CD3D-1866H | Active Recombinant Human CD3E & CD3D protein, His-tagged, HRP Labeled | +Inquiry |
CD3E & CD3D-2973H | Active Recombinant Human CD3E & CD3D protein, Fc-His &Fc-Flag-tagged, Biotinylated | +Inquiry |
Not For Human Consumption!
Inquiry
- Reviews
- Q&As
Ask a Question for All CD3E;CD3D Products
Required fields are marked with *
My Review for All CD3E;CD3D Products
Required fields are marked with *
0
Inquiry Basket