Active Recombinant Canine PDCD1 protein(Met1-Leu169), hFc-tagged

• Cat.No.: PDCD1-1057C
• Product Overview: Recombinant Canine PDCD1 (XP_543338.3) (Met1-Leu169) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Canine
• Tag: Fc
• Protein length: Met1-Leu169
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized PD-1 Protein, Canine, Recombinant (hFc Tag) at 2 μg/mL (100 μL/well) can bind PD-L1 Protein, Canine, Recombinant (hFc Tag), Biotinylated, the EC50 is 300-1200 ng/mL.
• Molecular Mass: The recombinant Canine PDCD1 consists 383 amino acids and predicts a molecular mass of 43 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.