Recombinant Human PDCD1, His-tagged, Biotinylated

• Cat.No.: PDCD1-257H
• Product Overview: Human PD-1 /PDCD1 Protein, Biotinylated (Biotinylated PD-1 / PDCD1) Pro 21 - 167 Gln, was labeled with biotin based on rhPD1 Protein, which was produced in human 293 cells (HEK293).

Specification

• Description: This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
• Molecular Mass: Biotinylated PD-1 / PDCD1, fused with 6×His tag at the C-terminus, has a calculated MW of 17.3 kDa. The predicted N-terminus is Pro 21. DTT-reduced Protein migrates as 38-42 kDa due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the Biotinylated PD-1 / PDCD1 by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
• Protein length: 21-167 a.a.

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Homo sapiens ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l
• Gene ID: 5133
• mRNA Refseq: NM_005018
• Protein Refseq: NP_005009
• MIM: 600244
• UniProt ID: Q15116
• Chromosome Location: 2q37.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; PD-1 signaling, organism-specific biosystem; T cell receptor signaling pathway, organism-specific biosystem
• Function: protein binding; protein tyrosine phosphatase activity; signal transducer activity