Active Recombinant Canine BMPR1A protein(Met1-Arg152), hFc-tagged

• Cat.No.: BMPR1A-1043C
• Product Overview: Recombinant Canine BMPR1A (NP_001138622.1) (Met1-Arg152) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Canine
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Arg152
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit rhBMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.5-2.5 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
• Molecular Mass: The recombinant Canine BMPR1A consists 370 amino acids and predicts a molecular mass of 41.2 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.