Active Recombinant B. pertussis Adenylate Cyclase Protein, His tagged

• Cat.No.: CYAA-01B
• Product Overview: Active Recombinant B. pertussis Adenylate Cyclase Protein (Gln2-Arg399) with an N-terminal Met and 6-His tag was expressed in E. coli.

Specification

• Species: B.pertussis
• Source: E.coli
• Tag: His
• ProteinLength: 2-399 aa
• Description: B. pertussis Adenylate Cyclase is an enzymatic toxin that converts ATP to 3''-5''cyclic AMP and plays a role in the pathogenesis of whooping cough. The Km for ATP of the enzyme is 0.6 mM. The enzyme activity is calmodulin (CaM) dependent with a Kd for CaM of 0.2 nM. The protein is synthesized as a large bifunctional precursor of 1706 amino acid residues, endowed with Adenylate Cyclase and haemolytic activity. The enzyme corresponds to amino acid 1 to 399 and is released from the precursor as a 43 kD fragment. The N-terminal portion (residues 1-235/237) harbors the catalytic site, whereas the C-terminal portion (residues 235/237-399) corresponds to the main CaM-binding domain of the enzyme
• Tag: His
• Molecular Mass: 44 kDa
• N-terminal Sequence Analysis: Met
• Bio-Activity: Measured by its ability to convert ATP to cAMP and pyrophosphate. The specific activity is > 250 nmol/min/μg
• Endotoxin: < 0.01 EU/μg by LAL
• Purity: > 90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain at 5 μg per lane.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Shipping: The product is shipped with polar packs.
• Reference: 1. Weiss, A.A. and E.L Hewlett (1986) Annu. Rev. Microbiol. 40:661.
  2. Glaser, P. et al. (1989) EMBO J.8:967.
  3. Shattuck, R.L. et al. (1985) Biochemistry 24:6358.

Gene Information

• Gene Name: cyaA bifunctional adenylate cyclase toxin/hemolysin CyaA [ Bordetella pertussis ]
• Official Symbol: cyaA
• Synonyms: cyaA; bifunctional adenylate cyclase toxin/hemolysin CyaA; bifunctional adenylate cyclase toxin/hemolysin CyaA
• Gene ID: 69600712
• Protein Refseq: WP_010929995
• UniProt ID: P15318