Active Recombinant 2019-nCoV ME Protein, His-Tagged

• Cat.No.: ME-270V
• Product Overview: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Methyltransferase / ME (YP_009724389.1) (Ser6799-Asn7096) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: Sars-Cov-2
• Source: E.coli
• Tag: His
• ProteinLength: Ser6799-Asn7096
• Description: Coronavirus encodes the 2′-O-MTase（2'O Methyltransferase） that is composed of the catalytic subunit nsp16 and the stimulatory subunit nsp10 and plays an important role in virus genome replication and evasion from innate immunity during viral infection. Nonstructural protein 16 (NSP16) / viral 2'O-methyltransferase (2'O-MTase) is highly conserved. The conserved 2'O-MTase activity is important for CoV pathogenesis and NSP16 is a conserved universal target for rapid live attenuated vaccine design in an expanding Coronavirus outbreak setting, such as COVID-19. Targeting on the 2'O-methylation pathway on SARS-CoV replication and pathogenesis can be the treatment options for vaccine and anti-viral durgs development which can against SARS-CoV-2，SARS-CoV, MERS-CoV or other RNA and DNA viruses.
• Predicted N Terminal: Met
• Form: Supplied as sterile 10mM Tris 250mM NaCl, 50% Glycerol pH 7.4.
• Bio-activity: Testing in progress
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Methyltransferase / ME consists of 299 amino acids and predicts a molecular mass of 33.46 kDa.
• Endotoxin: Please contact us for more information.
• Purity: > 85 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
• Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: Liquid. It is shipped out with blue ice.

Gene Information

• Gene Name: ORF1ab ORF1a polyprotein;ORF1ab polyprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: ORF1ab
• Synonyms: Methyltransferase, ME
• Gene ID: 43740578
• Protein Refseq: YP_009724389.1
• UniProt ID: P0DTC1