Active Native Phaseolus vulgaris (red kidney bean) Lectin

• Cat.No.: Lectin-8983P
• Product Overview: PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
  PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
  EC Number: 232-718-7
  NACRES: NA.32
  impurities: salt, free

Specification

• Description: PHAs are carbohydrate-binding proteins. There are several types of PHAs identified, predominantly in leguminous plants.
  Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found in plants (esp. in legumes). It is obtained from Phaseolus vulgaris (red kidney beans) and acts as a mitogen in human leucocyte culture.
• Source: Red kidney bean
• Species: Phaseolus vulgaris
• Form: lyophilized powder
• Bio-activity: ≤62.5 μg per mL agglutination activity
  ≤10 μg/mL mitogenic activity
• Applications: It has also been used as an antigen to measure in vivo T-cell-mediated immune responsiveness.
  Lectin from Phaseolus vulgaris (red kidney bean) has been used in phytohemagglutinin (PHA) assay and during assessment of cellular and humoral immunity.
  Agglutination activity is expressed in mg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 centigrade. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.
• Storage: 2-8 centigrade
• Protein length: 232-718 a.a.

Gene Information

• Official Symbol: PHA
• Synonyms: Phytohemagglutinin PHA-P; Lectin; PHA; Phaseolus vulgaris agglutinin