Active Native Human C5b,6 Complex Protein

• Cat.No.: C5b6-1537H
• Product Overview: Native Human C5b,6 Complex Protein was purified from normal human serum.

Specification

• Species: Human
• Source: Human Serum
• Tag: Non
• Description: C5b,6 is a product of complement activation of C5 and an intermediate in the formation of the C5b-9 membrane attack complex (MAC) of complement.
• Form: Frozen liquid
• Bio-activity: >10,000 Units/mg for lysis of sheep erythrocytes.
• Molecular Mass: 285,000 Da (3 chains). C5b,6 is composed of two disulfide linked chains from C5b (105,000 and 75,000) and one unlinked chain from C6 (105,000).
• Purity: >90% by SDS-PAGE
• Unit Definition: One unit of lytic activity is defined as the amount of C5b,6 complex required to yield 50% lysis of 1.5x10^7 sheep erythrocytes when incubated for 30 minutes at 37 centigrade in a total reaction volume of 75 µL GVBE (10 mM EDTA) containing 500 ng C7, 500 ng C8, and 1000 ng C9.
• Storage: Store at -70 centigrade or below. Avoid freeze/thaw.
• Storage buffer: 10 mM HEPES, 120 mM NaCl, pH 7.2

Gene Information

• Official Symbol: C5b,6 Complex