Active Native Griffonia Simplicifolia Lectin II Protein, Biotinylated

• Cat.No.: Lectin-1788G
• Product Overview: This product is the Biotinylated Griffonia Simplicifolia Lectin II and has sugar specificity against N-Acetylglucosamine.

Specification

• Species: Griffonia Simplicifolia
• Source: Griffonia Simplicifolia
• Tag: Non
• Description: This lectin is a dimeric glycoprotein composed of two subunits of nearly identical size with each subunit having disulfide-linked chains and a binding site for α- or β-linked N-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides.
• Bio-activity: Inhibiting/Eluting Sugar: Chitin Hydrolysate or 200 mM N-acetylglucosamine
• Molecular Mass: 113 kDa
• Applications: Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
• Storage: Refrigerate; for long term storage, aliquots may be stored frozen.
• Reconstitution: Reconstitute biotinylated lectin by addition 1ml of water. The resulting solution will have the following composition: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM Ca2+. For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer

Gene Information

• Synonyms: Lectin; GSL II; BSL II
• UniProt ID: Q41263