Active Native Clostridium difficile Toxin B Protein

• Cat.No.: TcdB-189C
• Product Overview: Native Toxin B purified from Clostridium difficile.

Specification

• Species: Clostridium difficile
• Source: Clostridium difficile
• Description: Toxin B from Clostridium difficile, also known as TcdB, is a cytotoxin that has glucosyltransferase and cysteine protease activities. It inactivates host Rho family GTPases by glucosylation, altering cytoskeletal structure, cell division, and cell-cell adhesion. Toxin B can induce activation of the inflammasome and apoptosis. While both toxin A and toxin B inactivate Rho family GTPases, toxin B more potently induces mammalian cell rounding in culture.
• Bio-activity: EC50 of 1 pg/mL as tested on Vero cells.
• Purity: ≥90% estimated by SDS-PAGE
• Storage: 2-8 centigrade (as supplied)
• Concentration: 0.2 mg/mL
• Storage Buffer: 50 mM Tris, 100 mM NaCl, pH 7.5

Gene Information

• Synonyms: TcdB; Toxin B
• UniProt ID: P18177