Active Native Bovine Myosin Protein (Full Length)

• Cat.No.: Myh2-20B
• Product Overview: Myosin protein has been purified from bovine cardiac muscle. The full length myosin protein has been purified with its essen-tial light chains (ELC) and regulatory light chains (RLC). Myosin has been determined to be biologically active in an F-actin activated ATPase assay. Bovine cardiac myosin protein is supplied as a white lyophilized powder.

Specification

• Description: Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified.
• Source: Cardiac Muscle
• Species: Bovine
• Form: White lyophilized powder
• Bio-activity: The biological activity of bovine cardiac myosin can be determined from its rate of F-actin activated ATP hydrolysis. A standard biological assay for monitoring ATP hydrolysis by myosin consists of an in vitro F-actin stimulated myosin ATPase assay. Strin-gent quality control ensures that in the presence of F-actin, bovine cardiac myosin will have a minimum ATP hydrolysis rate 10 fold greater than in the absence of F-actin and a minimum actin acti-vated ATPase activity > 25 nmol/min/mg of myosin.
• Protein length: Full Length
• Purity: Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20 % gradient polyacrylamide gel. Myosin protein is 95 % pure.
• Applications: Drug discovery target in cardiac research
  Measurement of F-actin activated myosin ATPase activity
  Identification/characterization of proteins or small molecules that affect myosin II ATPase activity
  Identification/characterization of proteins or small molecules that affect the myosin II - F-actin interaction
  Control myosin ATPase activity in drug discovery screening projects
• Stability: The lyophilized protein is stable at 4 centigrade desiccated (<10 % humidity) for 1 year.
• Storage: Briefly centrifuge to collect the product at the bottom of the tube. In order to maintain high biological activity of the protein, it is recommended that the protein solution be aliquoted into "experiment sized" amounts, snap frozen in liquid nitrogen and stored at -70 centigrade. The protein is stable for 6 months if stored at -70 centigrade. The protein should not be exposed to repeated freeze-thaw cycles. The lyophilized protein is stable at 4 centigrade desiccated (< 10 % humidity) for 1 year.
• Storage Buffer: 25 mM PIPES pH 7.0, 1.25 M KCl, 2.5 % (w/v) sucrose and 0.5 % (w/v) dextran.
• Reconstitution: The protein should be reconstituted to 10 mg/mL by the addition of 100 μL of Milli-Q water.

Gene Information

• Gene Name: MYH2 myosin heavy chain 2 [ Bos taurus (cattle) ]
• Official Symbol: MYH2
• Synonyms: MYH2; myosin heavy chain 2; MyHC-2a; myosin-2; myosin, heavy chain 2, skeletal muscle, adult; myosin, heavy polypeptide 2, skeletal muscle, adult; type 2a myosin heavy chain
• Gene ID: 788772
• mRNA Refseq: NM_001166227
• Protein Refseq: NP_001159699
• UniProt ID: Q9BE41