Active Native Rabbit Myosin II Protein

• Cat.No.: Myh2-13R
• Product Overview: Native rabbit full length myosin motor protein was from rabbit skeletal muscle. The full length myosin II protein has been purified with its essential light chains (ELC) and regulatory light chains (RLC).

Specification

• Species: Rabbit
• Source: Rabbit skeletal muscle
• Tag: Non
• Description: Muscle contraction. Required for cytoskeleton organization.
• Form: White lyophilized powder
• Bio-activity: The biological activity of rabbit myosin II is determined from its rate of F-actin activated ATP hydrolysis. A standard biological assay for monitoring ATP hydrolysis by myosin consists of an in vitro F-actin ATPase assay. Stringent quality control ensures that in the presence of F-actin, rabbit myosin will have a minimum hydrolysis rate 10 fold greater than in the absence of F-actin and a minimum actin activated ATPase activity >300 nmol/min/mg of myosin.
• Purity: Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% gradient polyacrylamide gel. Myosin protein is 95% pure
• Applications: Measurement of F-actin activated myosin ATPase activity
  Identification/characterization of proteins or small molecules that affect myosin II ATPase activity
  Identification/characterization of proteins or small molecules that affect the myosin II - F-actin interaction
  Control myosin ATPase activity in drug discovery screening projects
  Myosin II has been determined to be biologically active in an F-actin activated ATPase assay. Rabbit skeletal muscle myosin II is not recommended for use in motility assays.
• Storage: The lyophilized protein is stable at 4 centigrade desiccated (<10% humidity) for 1 year.
• Reconstitution: When reconstituted in nanopure water, the protein will be in the following buffer: 100 mM Imidizole pH 7.5, 2.4 M KCl, 0.2 mM DTT, 5% sucrose and 1% dextran.
• Full Length: Full L.

Gene Information

• Gene Name: MYH2
• Official Symbol: MYH2 myosin, heavy chain 2, skeletal muscle, adult [ Oryctolagus cuniculus (rabbit) ]
• Synonyms: MYH2; myosin, heavy chain 2, skeletal muscle, adult; myosin, heavy chain 2, skeletal muscle, adult; myosin, heavy polypeptide 2, skeletal muscle, adult
• Gene ID: 100009054