Active GMP Recombinant Human SHH Protein

• Cat.No.: SHH-01HG
• Product Overview: Recombinant human SHH protein, was expressed in CHO-K1 cell-derived in an animal component free process under cGMP guidelines.

Specification

• Species: Human
• Source: CHO
• Description: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities.
• Form: Lyophilized powder
• Endotoxin: <20 EU/mg
• Bio-activity: >1.0 x 10^6 IU/mg
• Purity: >95.0% by high performance liquid chromatography (SEC-HPLC) and SDS-PAGE.
• Storage Buffer: 10mM Phosphate-Buffered Saline (pH 7.2)
• Storage: Stable storage at 4℃ for 7-10 days; for long-term storage, please store at -20℃ after dispensing.
• Sterility: Sterility
• Instructions: It is recommended that lyophilized GMP rhSHH be dissolved in water for injection or sterilized ultrapure water at a concentration of not less than 100 μg/ml, pending further dilution to working concentration.

Gene Information

• Gene Name: SHH sonic hedgehog signaling molecule [ Homo sapiens (human) ]
• Official Symbol: SHH
• Synonyms: TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5
• Gene ID: 6469
• mRNA Refseq: NM_000193.4
• Protein Refseq: NP_000184.1
• UniProt ID: Q15465
• MIM: 600725