Active GMP Recombinant Human SHH Protein

• Cat.No.: SHH-111HG
• Product Overview: Recombinant Human SHH, Cys24-Gly197, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.

Specification

• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and DTT.
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is <5 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 20 kDa
  SDS-PAGE: 22 kDa, reducing conditions
• N-terminal Sequence Analysis: (Cys24)-Gly-Pro-Gly-Arg-Gly-Phe-Gly-Lys-Arg
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 250 μg/mL in sterile deionized water.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
• Tag: Non
• Protein length: 24-197 a.a.

Gene Information

• Gene Name: SHH sonic hedgehog [ Homo sapiens ]
• Official Symbol: SHH
• Synonyms: SHH; sonic hedgehog; sonic hedgehog protein; HHG1; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog homolog; HLP3; HPE3
• Gene ID: 6469
• mRNA Refseq: NM_000193
• Protein Refseq: NP_000184
• MIM: 600725
• UniProt ID: Q15465