Active GMP Recombinant Human NTF3 Protein

• Cat.No.: NTF3-118HG
• Product Overview: Recombinant Human NTF3, Tyr139-Thr257, was produced in Sf 21 (baculovirus)-derived under cGMP guidelines.

Specification

• Source: Insect cell
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 1-10 ng/mL.
  The specific activity of Recombinant Human NT-3 is approximately 2.1 x 10^3 units/μg, which is calibrated against recombinant human NT-3 Standard (NIBSC code: 98/718).
• Molecular Mass: Predicted Molecular Mass: 13.7 (monomer) kDa
• N-terminal Sequence Analysis: Tyr-Ala-Glu-His-Lys-Ser-His-Arg-Gly-Glu
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
• Tag: Non
• Protein length: 139-257 a.a.

Gene Information

• Gene Name: NTF3 neurotrophin 3 [ Homo sapiens ]
• Official Symbol: NTF3
• Synonyms: NTF3; neurotrophin 3; neurotrophin-3; NGF2; NT-3; NT3; HDNF; NGF-2
• Gene ID: 4908
• mRNA Refseq: NM_001102654
• Protein Refseq: NP_001096124
• MIM: 162660
• UniProt ID: P20783