Active GMP Recombinant Human IL12 Protein

• Cat.No.: IL12-160HG
• Product Overview: Recombinant p35 subunit of human IL-12, termed as IL12A (P29459.2) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; Recombinant p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified in an animal component free process under cGMP guidelines.

Specification

• Species: Human
• Source: HEK293
• Predicted N Terminal: Arg 23 & Ile 23
• Form: Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5 % mannitol and 0.01% Tween80.
• Bio-activity: Measured in a cell proliferation assay using anti-CD3 antibody activated human peripheral blood mononuclear cell (PBMC). The ED50 for this effect is typically 0.4-2 ng/ml. The specific activity of recombinant human IL-12 is approximately 1.3 x 10^4 units/μg, which is calibrated against recombinant human IL-12 WHO Standard (NIBSC code: 95/544).
• Molecular Mass: The GMP recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.2 (24 + 36.2) kDa.
• Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: IL12A interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) [ Homo sapiens ]
• Official Symbol: IL12A
• Synonyms: IL12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); NKSF1; interleukin-12 subunit alpha; CLMF; cytotoxic lymphocyte maturation factor 1; p35; IL 12; subunit p35; IL 12A; IL35 subunit; interleukin 12; interleukin 12 alpha chain; natural killer cell stimulatory factor 1; 35 kD subunit; NF cell stimulatory factor chain 1; NFSK; CLMF p35; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; natural killer cell stimulatory factor 1, 35 kD subunit; P35; CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF; NKSF2; P35
• Gene ID: 3592
• mRNA Refseq: NM_000882
• Protein Refseq: NP_000873
• MIM: 161560
• UniProt ID: P29459