Active GMP Recombinant Human FLT3LG Protein

• Cat.No.: FLT3LG-105HG
• Product Overview: Recombinant Human FLT3LG, Thr27-Pro185, was produced in Sf 9 (baculovirus)-derived in an animal component free process under cGMP guidelines.

Specification

• Source: Insect cell
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is 0.2-1 ng/mL.
  The specific activity of recombinant human Flt-3 Ligand is approximately 1.2 x 10^3 U/μg, which is calibrated against recombinant human Flt-3 Ligand WHO Standard (NIBSC code: 96/532).
• Molecular Mass: Predicted Molecular Mass: 17.5 kDa
  SDS-PAGE: 17-30 kDa, reducing conditions
• N-terminal Sequence Analysis: Thr-Gln-Asp-(Cys)-Ser-Phe-Gln-His-Ser-Pro
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining, under reducing
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100-200 μg/mL in PBS.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
• Tag: Non
• Protein length: 27-185 a.a.

Gene Information

• Gene Name: FLT3LG fms-related tyrosine kinase 3 ligand [ Homo sapiens ]
• Official Symbol: FLT3LG
• Synonyms: FLT3LG; fms-related tyrosine kinase 3 ligand; flt3 ligand; FL; FLT3L
• Gene ID: 2323
• mRNA Refseq: NM_001204502
• Protein Refseq: NP_001191431
• MIM: 600007
• UniProt ID: P49771