Active Recombinant Human FLT3LG Protein, Pre-aliquoted

• Cat.No.: FLT3LG-23H
• Product Overview: Recombinant Human FLT3LG Protein, Thr27-Pro185, was expressed in Insect cell.

Specification

• Species: Human
• Source: Insect Cells
• Protein Length: Thr27-Pro185
• Description: Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).
• Bio-activity: Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is 0.2-1 ng/mL.
• Molecular Mass: Predicted Molecular Mass:17.5 kDa
  SDS-PAGE: 17-30 kDa, reducing conditions
• N-terminal Sequence Analysis: Thr27
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 97% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll Pre-aliquoted directly into cell culture medium for immediate use.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: FLT3LG fms related receptor tyrosine kinase 3 ligand [ Homo sapiens (human) ]
• Official Symbol: FLT3LG
• Synonyms: FLT3LG; fms related receptor tyrosine kinase 3 ligand; FL; FLG3L; FLT3L; fms-related tyrosine kinase 3 ligand; flt3 ligand; fms related tyrosine kinase 3 ligand
• Gene ID: 2323
• mRNA Refseq: NM_001459
• Protein Refseq: NP_001450
• MIM: 600007
• UniProt ID: P49771