Acitve Recombinant Cynomolgus IL4R protein(Met1-Arg232), hFc-tagged

• Cat.No.: IL4R-1382C
• Product Overview: Recombinant Cynomolgus IL4R (EHH60265.1) (Met1-Arg232) was expressed in HEK293 with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: Fc
• Protein length: Met1-Arg232
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to inhibit IL-4 dependent prolifeRation of TF-1 Human erthroleukemic cells. The ED50 for this effect is 4-16 ng/ml in the presence of 0.2 ng/mL IL-4.2. Immobilized Recombinant Human IL4 / Interleukin-4 Protein at 2μg/mL (100μL/well) can bind Recombinant Cynomolgus IL4R Protein (ECD, Fc Tag), the EC50 is 18-56 ng/mL.
• Molecular Mass: The recombinant Cynomolgus IL4R consists of 445 amino acids and predicts a molecular mass of 50.48 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: IL4R