TR-FRET ROR alpha Coactivator Assay Kit, rabbit

• Cat.No.: Kit-0965

Specification

• Description: The TR-FRET Retinoid-related Orphan Receptor alpha (ROR alpha) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ROR alpha ligands as agonists or inverse agonists of coactivator recruitment. The homogeneous mix-and-read assay uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a ROR alpha ligand-binding domain (ROR alpha-LBD) that is tagged with glutathione-S-transferase (GST).
• Applications: Nuclear Receptor Assay
• Storage: Tb-anti-GST Antibody (Rabbit): Store at -20 °C
  ROR alpha-LBD, GST: Store at -80 °C
  Fluoroscein Peptide: Store at -20 °C
  TR-FRET Coregulator Buffer D: Store at 4 °C
  1M DTT: Store at -20 °C or -80 °C
• Size: 800 x 20 µL assays
• Materials Required but Not Supplied: Microplate Reader
• Detection method: Fluorescent
• Tag: Non