SNRNP70
-
Official Full Name
small nuclear ribonucleoprotein 70kDa (U1) -
Overview
snRNP70 also known as U1 small nuclear ribonucleoprotein 70 kDa is a protein that in humans is encoded by the SNRNP70 gene. snRNP70 is a small nuclear ribonucleoprotein that associates with U1 spliceosomal RNA, forming part of the spliceosome. -
Synonyms
SNRNP70;small nuclear ribonucleoprotein 70kDa (U1);RNPU1Z, RPU1, small nuclear ribonucleoprotein 70kDa (RNP antigen) , SNRP70;U1 small nuclear ribonucleoprotein 70 kDa;Snp1;U1 70K;U1 snRNP 70 kDa;RPU1;U1AP;U170K;U1RNP;RNPU1Z;SNRP70;U1-70K
Recombinant Proteins
- Human
- Zebrafish
- Rhesus macaque
- Mouse
- E.coli
- Mammalian Cell
- HEK293
- HEK293T
- Mammalian cells
- CHO
- Non
- His
- Myc&DDK
- Flag
- His&Avi
- His&Fc&Avi
Background
What is snrnp70 protein?
The snRNP70 protein, also known as U1-70K or U1 snRNP-specific B' protein, is a key component of the spliceosome complex. The spliceosome is responsible for removing introns (non-coding regions) from pre-mRNA during the process of RNA splicing.
snRNP70 specifically binds to the U1 small nuclear RNA (snRNA) molecule, which is part of the U1 small nuclear ribonucleoprotein (snRNP) complex. This binding is crucial for the recognition of the 5' splice site of pre-mRNA, initiating the splicing process.
The snRNP70 protein consists of two functional domains: an N-terminal RNA-binding domain that interacts with U1 snRNA, and a C-terminal protein-protein interaction domain that helps in the assembly of the spliceosome. It plays a vital role in maintaining the stability and function of the U1 snRNP complex, ensuring accurate splicing of pre-mRNA.
What is the function of snrnp70 protein?
The snRNP70 protein, also known as U1 small nuclear ribonucleoprotein 70 kDa, is a component of the spliceosome complex involved in pre-mRNA splicing. The spliceosome is responsible for removing introns and joining exons to produce mature mRNA molecules. snRNP70 is specifically associated with the U1 small nuclear ribonucleoprotein particle, which recognizes the 5' splice site of introns during spliceosome assembly. It plays a crucial role in the recognition and binding of the splice site, ultimately facilitating the accurate and efficient splicing of pre-mRNA. This process is essential for gene expression and the production of functional proteins in eukaryotic cells.
Snrnp70 related signaling pathway
The activation and regulation of the spliceosome are complex processes that involve various signaling events and post-translational modifications of spliceosome components. Several signaling pathways, such as the MAPK/ERK and PI3K/AKT pathways, have been implicated in the regulation of alternative splicing. These pathways can regulate the activity and expression of splicing factors, including Snrnp70, to modulate splicing patterns and ultimately control gene expression.
Snrnp70 Related Diseases
The snRNP70 protein has several functions in RNA splicing. It binds to the U1 snRNA and helps in the recognition of the 5' splice site sequence. It also interacts with other snRNP proteins and spliceosome components, facilitating the assembly of the spliceosome complex.
Moreover, the snRNP70 protein plays a role in regulating alternative splicing events. It can modulate the selection of different splice sites, contributing to the generation of multiple transcript isoforms from a single gene.
Defects or mutations in the snRNP70 protein can lead to abnormal RNA splicing, resulting in various diseases. For example, mutations in the snRNP70 gene have been associated with autoimmune diseases such as systemic lupus erythematosus (SLE), where the immune system mistakenly attacks healthy tissues.
Biomedical Application of snrnp70 Protein
Autoimmune diseases: Some individuals with systemic lupus erythematosus (SLE) or mixed connective tissue disease (MCTD) develop autoantibodies against snRnp70. These autoantibodies can interfere with the function of snRNP complexes, leading to impaired pre-mRNA splicing and contributing to the pathogenesis of these autoimmune diseases.
Splicing-related diseases: Mutations in genes encoding components of the splicing machinery, including snRnp70, can lead to various splicing-related diseases. For instance, mutations in snRnp70 have been associated with retinitis pigmentosa, a hereditary eye disease characterized by progressive vision loss.
Case Study
(AMIT K. SINGH, 2023)
Fig2. Proximity of cohesin to U1-70 increases in the absence of BRD4.PLAs between anti-RAD21/anti–U1-70 antibodies following the indicated treatment conditions.
(Alejandro Ibáñez-Costa, 2022)
Fig3. In vitro, the induced overexpression of KHDRBS1 and SNRNP70 promote the downregulation of key inflammatory mediators and functional endpoints in RA. Monocytes, lymphocytes and neutrophils from patients with RA were transiently transfected using KHDRBS1 and SNRNP70 plasmid and empty vector (mock) used as control. Protein expression of KHDRBS1 and SNRNP70 after transfection by Western Blot analysis.
(Nikolas Nikolaou, 2022)
Fig4. A representative image of a dissociated motor neuron at 1 day in vitro derived from Tg(mnx1:GFP) animals at 28 hpf. The sample was stained with anti-SNRNP70 to reveal the localization of the protein in neurites and DAPI to mark nuclei. Scale bars, 10 μm.
Involved Pathway
SNRNP70 involved in several pathways and played different roles in them. We selected most pathways SNRNP70 participated on our site, such as Spliceosome, which may be useful for your reference. Also, other proteins which involved in the same pathway with SNRNP70 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Spliceosome | SYF2,PRPF3,SRSF1,SNRPG,SNRPA1,SNRPF,HSPA2,PRPF38A,CDC5L,ALYREF |
Protein Function
SNRNP70 has several biochemical functions, for example, RNA binding,U1 snRNA binding,mRNA binding. Some of the functions are cooperated with other proteins, some of the functions could acted by SNRNP70 itself. We selected most functions SNRNP70 had, and list some proteins which have the same functions with SNRNP70. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| RNA binding | SETD1B,HNRNPA1L2,PABPN1L,RC3H2,CSTF2,XPO5,CTU1,CNBPA,RPL15,PABPC1A |
| protein binding | KIAA1143,UROD,YAF2,DERL1,CNTROB,GTF2H2C,PSMA7,GSTP1,DNMBP,TUBB |
| nucleotide binding | TDRD9,ABCB6,MAPKAPK2B,KIF20B,ABCC12,GKUP,HNRNPAB,GATB,NEK4,MXG |
| U1 snRNA binding | ISG20,SNRPA,PRPF8 |
| poly(A) RNA binding | KHDRBS1,HNRNPAB,FIP1L1,HSPE1,ILF3,RPS23,AKAP8,TRIP6,MSI1,PEG10 |
| mRNA binding | PIWIL2,RBM5,TPR,CLUHA,SOSTDC1B,FYTTD1,NUDT21,UHMK1,RBMY1A1,PCF11 |
Interacting Protein
SNRNP70 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with SNRNP70 here. Most of them are supplied by our site. Hope this information will be useful for your research of SNRNP70.
SRPK2;RNPS1;VCAM1;CLK2;SRPK1;WBP4;MYC;SFPQ;GSK3B;SF3A2;PRMT5;SMAD9;PRPF40A
Resources
Related Services
Related Products
References
