CDK8
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Official Full Name
cyclin-dependent kinase 8 -
Overview
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members;are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are;known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are;components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the;largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the;CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the;Mediator-like protein complexes and the basal transcription machinery. -
Synonyms
CDK8;cyclin-dependent kinase 8;K35;protein kinase K35;CDK8 protein kinase;mediator complex subunit CDK8;cell division protein kinase 8;mediator of RNA polymerase II transcription subunit CDK8;MGC126074;MGC126075
Recombinant Proteins
- Human
- Mouse
- Rhesus macaque
- Zebrafish
- E.coli
- Sf9 Insect Cell
- Insect Cell
- HEK293
- Wheat Germ
- Mammalian Cell
- Mamanlian cells
- HEK293T
- Insect (sf21)
- In Vitro Cell Free System
- Non
- His
- GST
- Myc&DDK
- His&Fc&Avi
- Flag
Background
What is cdk8 protein?
Cdk8, also known as cyclin-dependent kinase 8, is a protein that is involved in cell cycle regulation and transcriptional regulation. It is a member of the cyclin-dependent kinase (CDK) family, which are enzymes that control the progression of the cell cycle by phosphorylating target molecules.
What is the function of cdk8 protein?
Cdk8 functions as a component of the Mediator complex, which is involved in the regulation of transcription by bridging the interaction between transcription factors and RNA polymerase II. Cdk8 can phosphorylate various transcription factors, coactivators, and chromatin remodeling proteins, thereby modulating gene expression.
The activity of Cdk8 is not essential for basic cell cycle progression, but it has been implicated in the regulation of cellular processes such as development, differentiation, and oncogenesis. Furthermore, aberrant regulation of Cdk8 activity has been linked to various diseases and conditions, including cancer, cardiovascular diseases, and neurological disorders.
Cdk8 related signaling pathway
The exact signaling pathway of Cdk8 is not fully understood, but it is known to be involved in various cellular processes such as cell cycle progression, transcriptional regulation, and development. Cdk8 is believed to act as a kinase that phosphorylates and regulates the activity of other proteins involved in these signaling pathways.
Additionally, Cdk8 has been found to be associated with the Wnt/β-catenin signaling pathway, which plays a crucial role in embryonic development and tissue homeostasis. In this context, Cdk8 functions as a transcriptional coactivator, modulating the activity of β-catenin in regulating gene expression.
Cdk8 Related Diseases
- Cancer: Overexpression or mutations of CDK8 have been found in many cancers like prostate cancer, breast cancer, colorectal cancer etc. CDK8 promotes cancer cell proliferation and survival.
- Medulloblastoma: CDK8 mutations and amplifications lead to Wnt pathway dysregulation and development of this pediatric brain tumor.
- Congenital heart disease: Deletions or mutations involving CDK8 cause Tetralogy of Fallot and atrioventricular septal defects.
- Cancer therapy: Cdk8 is overexpressed in many types of cancer, including colorectal, breast, and prostate cancer. Inhibition of cdk8 activity can suppress tumor growth and progression, making it a potential target for cancer therapy.
- Regenerative medicine: Cdk8 is involved in the regulation of stem cell self-renewal and differentiation. Manipulating cdk8 activity can potentially enhance the regeneration and differentiation of stem cells, offering possibilities for regenerative medicine approaches.
- Metabolic disorders: Cdk8 has a role in metabolic regulation, including glucose and lipid metabolism. Targeting cdk8 may have potential applications in the treatment of metabolic disorders, such as diabetes and obesity.
Biomedical Application of cdk8 Protein
- Cancer therapy: Cdk8 is overexpressed in many types of cancer, including colorectal, breast, and prostate cancer. Inhibition of cdk8 activity can suppress tumor growth and progression, making it a potential target for cancer therapy.
- Drug discovery: Cdk8 inhibitors have been developed and studied as potential therapeutic agents. These inhibitors can selectively target cdk8 and its associated signaling pathways, providing opportunities for the development of novel drugs.
- Inflammatory diseases: Cdk8 has been implicated in the regulation of inflammatory pathways. Modulating cdk8 activity may have therapeutic benefits in the treatment of inflammatory diseases, such as rheumatoid arthritis and inflammatory bowel disease.
- Inflammatory diseases: Cdk8 has been implicated in the regulation of inflammatory pathways. Modulating cdk8 activity may have therapeutic benefits in the treatment of inflammatory diseases, such as rheumatoid arthritis and inflammatory bowel disease.
- Metabolic disorders: Cdk8 has a role in metabolic regulation, including glucose and lipid metabolism. Targeting cdk8 may have potential applications in the treatment of metabolic disorders, such as diabetes and obesity.
Case Study
(Xinli Qu, 2013)
Fig3. Analysis of Smad3 T388 phosphorylation and the role of T388 in Smad3 complex formation with Smad4 and CDK8. D: HEK293T cells transfected with Smad3 plasmids were analyzed by IP/WB for TGF-β1–induced complex formation between Smad3 and CDK8; graph shows the quantification.
(Ning Li, 2021)
Fig4. Expression of TA-CDK mRNAs in breast cancer (GEPIA2).
(Xiang-Jie Kong, 2015)
Fig5 The expression of miR-497 in PCa and the CCK-8 assay in PC3-AR cells. A. The relative expression of miR-497 in 20 pairs of serum samples from patients with PCa and healthy control subjects were analyzed by qRT-PCR. B. CCK-8 assay of PC3-AR cells transfection with miR-497, miR-NC, anti-miR-497, siR-IKKβ, and siR-NC. C. CCK-8 assay of PC3-AR cells treatment with IMD-0354 in different concentrations (μM).
Quality Guarantee
High Purity
Fig1. SDS-PAGE (CDK8-1038H)
High Bioactivity & Detection Sensitivity
Fig2. The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into streptavidin- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG.
Involved Pathway
CDK8 involved in several pathways and played different roles in them. We selected most pathways CDK8 participated on our site, such as Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants,Constitutive Signaling by NOTCH1 PEST Domain Mutants,Developmental Biology, which may be useful for your reference. Also, other proteins which involved in the same pathway with CDK8 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Fatty acid, triacylglycerol, and ketone body metabolism | ACOT7,DECR1,THEM5,SREBF2,CPT2,APOA5,GRHL1,TIAM2,AGT,ACSF2 |
| Gene Expression | CTDP1,POLRMT,ZNF750,TRMT61A,TH1L,AIMP2,METTL14,SF3B2,FAM36A,NR2F6B |
| Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants | JAG1,HDAC6,JAG2,HEYL,HEY1,HEY2,MIB1,CCNC,ADAM10,HDAC5 |
| Developmental Biology | TRPC3,CHL1,LGI2B,EFNA4,ACTR3,ANK2B,GPC1B,SEMA4D,JAK1,LGI2A |
| Diseases of signal transduction | HEYL,CTBP2,FGFR1OP,CDC37,HDAC5,JAG2,STRA6,RBP1,OPN1SW,LRRFIP1 |
| Constitutive Signaling by NOTCH1 PEST Domain Mutants | HEY1,HEYL,HEY2,ADAM10,JAG2,CCNC,HDAC6,HDAC5,MIB1,JAG1 |
| Disease | COBRA1,FGFR1OP2,TRIM24,NA,KPNA5,ARF1,KPNA3,TCEB3,GPC6,OGN |
| Generic Transcription Pathway | NR2E3,ZNF596,ZNF167,ESRRA,ZNF324,HIPK1,CTGF,ZNF669,ARHGEF38,COX6A1 |
Protein Function
CDK8 has several biochemical functions, for example, ATP binding,RNA polymerase II carboxy-terminal domain kinase activity,cyclin-dependent protein serine/threonine kinase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by CDK8 itself. We selected most functions CDK8 had, and list some proteins which have the same functions with CDK8. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| RNA polymerase II carboxy-terminal domain kinase activity | CDK7,CDK12,CDK1,CDK9,MAPK1,CCNK,CDK13 |
| protein kinase activity | ACVR1L,PLK1,RPS6KB1B,ANKK1,ROCK2B,MAP2K2A,CDK17,PLK5,PRPF4B,PRKCI |
| ubiquitin protein ligase activity | CCNB1IP1,BFAR,HUWE1,ZSWIM2,UBR2,ARIH1,RNF149,CDC42,MSL2B,UBE2KB |
| cyclin-dependent protein serine/threonine kinase activity | STK30,CDK20,MAK,CDK17,PFTK1,CDK15,CDK6,CDK16,CDK12,CDK11A |
| ATP binding | ATP11C,NLRP3,DALRD3,VPS4A,NEK1,GUCY2C,HSPA6,SLFN5,ABCC10,TTLL9 |
| protein binding | FAM154A,CCL28,BIN3,ATP6V1D,UBL4B,FUS,HTR6,ITGB1,SUN1,CST5 |
Interacting Protein
CDK8 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with CDK8 here. Most of them are supplied by our site. Hope this information will be useful for your research of CDK8.
CCNC
CDK8 Related Signal Pathway
Resources
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References
- Qu, XL; Li, XL; et al. Regulation of Renal Fibrosis by Smad3 Thr388 Phosphorylation. AMERICAN JOURNAL OF PATHOLOGY 184:944-952(2014).
- Rovnak, J; Quackenbush, SL; et al. Walleye dermal sarcoma virus cyclin interacts with components of the mediator complex and the RNA polymerase II holoenzyme. JOURNAL OF VIROLOGY 76:8031-8039(2002).
