Active Recombinant Full Length Human CDK8 Protein, DDK-tagged, Biotinylated

• Cat.No.: CDK8-2923HF
• Product Overview: Full-length human CDK8 [1-464(end) amino acids of accession number NP_001251.1] was co-expressed as N-terminal DYKDDDDK tagged, biotinylated protein (57 kDa) with human GST-CyclinC [1-283(end) amino acids of accession number NP_005181.2] using baculovirus expression system. The protein was purified by using DYKDDDDK tag antibody agarose.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: Flag
• Protein Length: 1-464(end)
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was determined by ELISA. The enzyme was incubated with biotinylated peptide in streptavidin-coated ELISA plate. Phosphorylation was detected by anti-phospho antibody and HRP- labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG).
• Purity: 98%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: cyclin-dependent kinase 8
• Official Symbol: CDK8
• Synonyms: CDK8;IDDHBA;K35
• Protein Refseq: NP_001251.1
• UniProt ID: P49336