Recombinant ZIKV(strain Zika SPH2015) E/Envelope protein, His-tagged

• Cat.No.: Envelope-569W
• Product Overview: Recombinant ZIKV(strain Zika SPH2015) E/Envelope (domain III) (ALU33341.1) (Val593-Lys699) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: Zika
• Source: HEK293
• Tag: His
• Protein Length: Val593-Lys699
• Description: Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH2015 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis.
• Predicted N Terminal: Val 593
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Zika virus (strain Zika SPH2015) E / Envelope (domain III) consists of 118 amino acids and predicts a molecular mass of 13 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Official Symbol: Envelope
• Synonyms: E/Envelope