Recombinant Zika virus (strain Zika SPH2015) Envelope protein (Domain I), His-MBP-tagged

• Cat.No.: Envelope-566Z
• Product Overview: Recombinant Zika virus (strain Zika SPH2015) E (ALU33341.1) (Ile291-Thr399 and Glu426-Gly485 and Ser576- Gly592), fused with a N-terminal polyhistidine-tagged MBP tag (his-MBP), was expressed in E. coli.

Specification

• Species: Zika
• Source: E.coli
• Tag: His&MBP
• Protein Length: 291-399;426-485;576-592 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 250 mM NaCl, 50 mM Tris, pH 8.0, 5% trehalose, 5% mannitol and 0.01% Tween80.
• Molecular Mass: The recombinant Zika virus (strain Zika SPH2015) E consists of 523 amino acids and predicts a molecular mass of 57.2 kDa.
• Purity: > 75 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Official Symbol: Envelope
• Synonyms: Envelope; E Protein
• Protein Refseq: ALU33341.1