Recombinant Urate Oxidase (Pseudogene)

• Cat.No.: UOX-84H
• Product Overview: Recombinant Urate Oxidase produced in E.Coli is a tetrameric, non-glycosylated polypeptide chain containing 301 amino acids, having a molecular formula of C1523H2383N417O462S7 and a molecular mass of 34,247 Dalton.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Urate oxidase also called uricase urate oxygen, oxidoreductase, catalyzes the enzymatic oxidation of uric acid into allantoin, an inactive and soluble metabolite.
• Amino Acid Sequence: msavkaaryg kdnvrvykvh kdektgvqtv yemtvcvlle geietsytka dnsvivatds ikntiyitak qnpvtppelf gsilgthfie kynhihaahv nivchrwtrm didgkphphs firdseekrn vqvdvvegkg idiksslsgl tvlkstnsqf wgflrdeytt lketwdrils tdvdatwqwk nfsglqevrs hvpkfdatwa tarevtlktf aednsasvqa tmykmaeqil arqqlietve yslpnkhyfe idlswhkglq ntgknaevfa pqsdpnglik ctvgrsslks kl.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Each 1.5 mg urate oxidase contains 5 mg sucrose, 25 mg glycine, 0.1 mg Tween-80, 13.6 mg Na2HPO4*12H20 and 0.33 mg NaH2PO4*2H20.
• Biological Activity: The specific activity was found to be 18 U/mg. One Unit oxidizes one micromole of uric acid per minute at 30°C, at pH 8.9.
• Solubility: It is recommended to reconstitute the lyophilized UOX in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Stability: Lyophilized UOX although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution UOX should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.