Recombinant Triticum aestivum Tri a 19 Allergen, His tagged, Biotinylated

• Cat.No.: Tri a 19-062T
• Product Overview: Tri a 19 or omega-5 gliadin, the major allergen of wheat, has been prepared as a recombinant mature allergen fused to a his-tag in its N-terminus and monobiotinylated in vivo.

Specification

• Species: Triticum aestivum
• Source: E.coli
• Tag: His
• Description: Tri a 19, or omega-5 gliadin, the major allergen of wheat, has been prepared as a recombinant mature allergen fused to a his-tag.
• Tag: N-His
• Conjugation/Label: Biotin
• Molecular Mass: Determined by SDS-PAGE, the protein band is between the molecular markers of 116 and 66.2 kDa, while relative molecular mass calculated from amino acid sequence is 68,614.82 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, PO
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 6, 0.15 M NaCl, 0.13 M trehalose, 0.1% polyoxyethylene (10) tridecyl ether and 1 M urea
• Concentration: 1.09 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Matsuo, H., Kohno, K., and F. Morita. Molecular cloning, recombinant expression and IgE-binding epitope of -5 gliadin, a major allergen in wheat-dependent exerciseinduced anaphylaxis. 2005, FEBS Journal 272: 4431–4438
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.