Recombinant Treponema pallidum Tpp47 Antigen, His&GST tagged, Biotinylated

• Cat.No.: Tpp47-060T
• Product Overview: The Tpp47 recombinant lipoprotein has been prepared by expressing the ORF of the corresponding gene which codifies the mature lipoprotein of 47 kDa of the spirochete Treponema pallidum and monobiotinylated in vivo.

Specification

• Species: Treponema pallidum
• Source: E.coli
• Tag: His&GST
• Description: Tpp47 is one of the major antigenic components of the bacterium Treponema pallidum. It triggers an early humoral response three to six days after infection. The Tpp47 antigen has been reported as a potential market for active syphilis disease, as anti-Tpp47 IgM have been detected in patients with congenital syphilis.
• Tag: N-His&GST
• Conjugation/Label: Biotin
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 116-66.2 kDa, while relative molecular mass calculated from amino acid sequence is 88,321.7 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, CE, DAS, NP, PO
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 8, 0.3 M NaCl, 5 mM EDTA and 0.1% polyoxyethylene (10) tridecyl ether
• Concentration: 1.25 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Ana Paula Félix de Miranda and Neuza Satomi Sato. Profile of Anti-Tp47 antibodies in patients with positive serology for syphilis analized by western blot. 2008, The Brazilian Journal of Infectious Diseases 12(2):139-143.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.