Recombinant Treponema pallidum 15 kDa Membrane Protein, GST tagged

• Cat.No.: TP15-11T
• Product Overview: Recombinant Treponema pallidum 15 kDa protein produced as a gst-fusion protein in the E. coli Expression System.

Specification

• Species: Treponema pallidum
• Source: E.coli
• Tag: GST
• Description: Treponema pallidum is a gram-negative spirochaete bacterium and is considered to be metabolically crippled. There are at least four known subspecies: T. pallidum pallidum， T. pallidum pertenue， T. pallidum carateum and T. pallidum endemicum. The helical structure of T. pallidum pallidum allows it to move in a corkscrew motion through viscous mediums such as mucus. Treponema pallidum sub sp. pallidum has one of the smallest bacterial genomes at 1.14 million base pairs （Mb） and has limited metabolic capabilities， reflecting its adaptation through genome reduction to the rich environment of mammalian tissue.
• Form: Frozen Liquid
• Molecular Mass: 42 kDa
• Purity: > 95%, this protein is purified by affinity chromatography and by preparative electrophoresis to > 95% purity as determined by SDS-PAGE, reduced.
• Applications: In-Vitro Diagnostics, Immunization
• Usage: Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100 ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100 ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2 mg/mL.
• Stability: 2 years at -75 centigrade
• Storage: At -75 centigrade
• Concentration: 1 mg/mL
• Dilutions: 10 mM Tris, pH 8.0, SDS (< 0.01%)

Gene Information

• Official Symbol: TP15
• Synonyms: TP15; T. pallidum TP15; Treponema pallidum 15kD Membrane Protein; Treponema pallidum p15