Recombinant Streptomyces Viridochromogenes PAT Protein (1-183 aa), His-SUMO-tagged

• Cat.No.: PAT-1945S
• Product Overview: Recombinant Streptomyces Viridochromogenes (strain DSM 40736/JCM 4977/BCRC 1201/Tue 494) PAT Protein (1-183 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Full Length.

Specification

• Species: Streptomyces Viridochromogenes
• Source: E.coli
• Tag: His&SUMO
• Protein Length: 1-183 aa
• Description: Inactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance.
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 36.6 kDa
• AA Sequence: MSPERRPVEIRPATAADMAAVCDIVNHYIETSTVNFRTEPQTPQEWIDDLERLQDRYPWLVAEVEGVVAGIAYAGPWKARNAYDWTVESTVYVSHRHQRLGLGSTLYTHLLKSMEAQGFKSVVAVIGLPNDPSVRLHEALGYTARGTLRAAGYKHGGWHDVGFWQRDFELPAPPRPVRPVTQI
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Synonyms: pat; Phosphinothricin-resistance protein
• UniProt ID: Q57146