Recombinant SARS Spike S1 protein, hFc-Avi-tagged

• Cat.No.: Spike-6733S
• Product Overview: Recombinant SARS Spike S1 protein(P59594)(Ser14-Arg667), fused with C-terminal hFc and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: SARS
• Tag: C-hFc-Avi
• Protein length: Ser14-Arg667
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized SARS Spike S1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, His Tag with the EC50 of 0.21μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 100.6 kDa. Due to glycosylation, the protein migrates to 120-140 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.