Active Recombinant COVID-19 BA.2.75.2 (Omicron) Spike RBD protein, His-tagged

• Cat.No.: Spike-803V
• Product Overview: Recombinant COVID-19 BA.2.75.2 (Omicron) Spike RBD protein(YP_009724390.1, with mutation G339H, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, G446S, N460K, S477N, T478K, E484A, F486S, Q498R, N501Y, Y505H) (Arg319-Phe541) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: COVID-19
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 (BA.2.75.2) Spike RBD Protein (His Tag), the EC50 is 20-60 ng/mL.
• Molecular Mass: The recombinant SARS-CoV-2 Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.63 kDa. As a result of glycosylation, it migrates as an approximately 35.78 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Arg319-Phe541
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: S
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2