Recombinant SARS-CoV(Beijing 02) S(ΔTM) protein

• Cat.No.: SARSS-101V
• Product Overview: Purified recombinant SARS-CoV(Beijing 02) S(ΔTM) protein was expressed in HEK293 cells.

Specification

• Species: SARS-CoV
• Source: HEK293
• Description: Spike protein S1: Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 and CLEC4M/DC-SIGNR receptors and internalization of the virus into the endosomes of the host cell induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.Spike protein S2: Mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.
• Form: Each vial contains 100 ug purified protein (1mg/ml) in PBS (pH7.4).
• Purity: >95%
• Applications: WB standard, Ab ELISA, immunogen, etc.
• Usage: For research use only, not for use in diagnostic procedures.
• Storage: Store at -80 centigrade, Avoid freeze/thaw cycle.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: S
• Official Symbol: S
• Synonyms: E2
• Gene ID: 1489668
• Protein Refseq: NP_828851.1
• UniProt ID: P59594