Recombinant SARS-CoV-2 ORF1ab Protein, His-tagged

• Cat.No.: ORF1ab-184S
• Product Overview: Active recombinant SARS-CoV-2 ORF1ab Protein (5325-5925) with a N-terminal His tag was expressed in E. coli.

Specification

• Species: SARS-CoV-2
• Source: E.coli
• Tag: His
• Protein Length: 5325-5925
• Description: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.
• Molecular Mass: 69.1 kDa
• Endotoxin: <1.0 EU/μg, determined by the LAL endotoxin assay
• Purity: ≥80% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to release 1 pmol of fluorescein-labeled ssDNA from a fork DNA substrate containing a quencher on the complimentary strand per minute at 25 centigrade under the following conditions: 20 mM HEPES, pH7.5, 20 mM NaCl, 5% glycerol, 5 mM MgCl2, 0.01% BSA, 2 mM DTT, 2 μM unlabeled complementary ssDNA, 1 mM ATP, and 200 nM forked DNA substrate.
• Stability: ≥ 1 year
• Storage: At 4 centigrade.
• Storage Buffer: 50 mM Tris-HCl, pH 7.5, with 5 μM zinc chloride, 1 mM DTT, 1 mM EDTA, and 10% glycerol

Gene Information

• Gene Name: ORF1ab ORF1a polyprotein;ORF1ab polyprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: ORF1ab
• Synonyms: ORF1ab; ORF1a polyprotein; ORF1ab polyprotein; leader protein; nsp2; nsp3; nsp4; 3C-like proteinase; nsp6; nsp7; nsp8; nsp9; nsp10; RNA-dependent RNA polymerase; helicase; 3'-to-5' exonuclease; endoRNAse; 2'-O-ribose methyltransferase; leader protein; nsp2; nsp3; nsp4; 3C-like proteinase; nsp6; nsp7; nsp8; nsp9; nsp10; nsp11; pp1ab; translated by -1 ribosomal frameshift; pp1a
• Gene ID: 43740578
• Protein Refseq: YP_009724389
• UniProt ID: P0DTD1