Active Recombinant COVID-19 ORF1ab protein, His-tagged

• Cat.No.: ORF1ab-0640C
• Product Overview: Recombinant COVID-19 ORF1ab protein(Glu746-Lys1060), fused with His tag, was expressed in E.coli.

Specification

• Species: Sars-CoV-2
• Source: E.coli
• Tag: His
• Protein Length: 746-1060 a.a.
• Form: Supplied as 0.2 μm filtered solution in 50 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH7.5 with glycerol as protectant.
• Bio-activity: Measured by its ability to convert the substrate Dabcyl–FRLKGGAPIKGV–Edans.The specific activity is >2500 pmol/min/mg.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 37.6 kDa. The protein migrates as 35-37 kDa under reducing (R) condition (SDS-PAGE).
• Proteinlength: Glu746-Lys1060
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.