Recombinant SARS-CoV-2 N Protein

• Cat.No.: N-501S
• Product Overview: Recombinant SARS-CoV-2 N Protein without tag was expressed in HEK293.

Specification

• Species: SARS-CoV-2
• Source: HEK293
• Description: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The nucleocapsid phosphoprotein is a structural protein that binds to, protects the viral RNA genome and is involved in packaging the RNA into virus particles. The N protein has been suggested as an antiviral drug target.
• Form: Liquid
• Molecular Mass: 47 kDa
• AA Sequence: MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method
• Purity: > 95% by SDS-PAGE
• Applications: ELISA, WB
• Storage: We recommended to store this recombinant protein as received at 2-8 centigrade for up to one month. For longer-term storage, aseptically aliquot in working volumes without diluting and store at -80 centigrade. Avoid Repeated Freeze Thaw Cycles.
• Concentration: 0.5 mg/mL
• Storage Buffer: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
• Shipping: Blue Ice via Next Day Air

Gene Information

• Gene Name: N nucleocapsid phosphoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: N
• Synonyms: SARS-CoV-2 N protein; Coronavirus NP; coronavirus Nucleocapsid; coronavirus Nucleoprotein; 2019-nCoV N protein Severe acute respiratory syndrome coronavirus 2 nucleocapsid; 100778-1; 100778-2; wild type; nucleocapsid phosphoprotein; ORF9; structural protein; N; nucleocapsid phosphoprotein
• Gene ID: 43740575
• Protein Refseq: YP_009724397